ABSTRACT
Objective To investigate the immunophenotypic characteristics of potential leukemia cells transfected with CD19 antigen receptor( CAR) during CAR-T cell preparation. Methods Morphological chan-ges in CD19 CAR-transfected cells were observed under inverted microscope. The transfection rate and immuno-phenotype of transfected Nalm-6 cells were analyzed by flow cytometry. Secretion of cytokines in the culture sys-tem was detected by chemiluminescence. Results The transfection rate of Nalm-6 cells by CD19 CAR was (46. 50±3. 78) ％ and that of KG1a cells was (15. 70±1. 22) ％. CD19 CAR-transfected Nalm-6 cells prolifer-ated more rapidly than Nalm-6 cells ( P values on 0 d, 4 d, 7 d and 12 d were 6. 339, 3. 447, 0. 012 and 0. 009). In the culture of CD19 CAR-transfected Nalm-6 cells, cell aggregation and adhesion were observed and they gradually gathered into a group. The rate of CD19 expression was only 1. 19％ in the CD19 CAR-transfect-ed Nalm-6 cell culture system with the transfection rate of (46. 50±3. 78) ％. After increasing the proportion of Nalm-6 cells in the culture system, CD19 expression was gradually increased, while the expression of CD22 re-mained stable. CD19 expressed by Nalm-6 cells cultured in the supernatant of CD19 CAR-transfected Nalm-6 cell culture system was decreased gradually. The levels of IL-10 and TNF-αsecreted by CD19 CAR-transfected Nalm-6 cells were higher than those by Nalm-6 cells. Conclusions Results of the immunophenotypic analysis of CD19 CAR-transfected leukemia cells suggested that CD22 CAR-T cell therapy could be used as a rescue or combination therapy for CD19 CAR transfection into leukemia cells.
ABSTRACT
Formation mechanism of breast cancer mainly includes genetic factors,diet and obesity,radioactive ray and ionizing radiation,menstrual,marriage,lactation factor and mental factors.Sympathetic nerve activity is mainly to ensure the needs of the physiology of the human body in a state of tension,while when sympathetic nerve function abnormal sustained and enhanced,the various systems of the body will receive adverse effects.For the plant nerve disorder,which can cause work stress,anxiety,worry and so on.With the further research of breast cancer in recent years,it shows that the change of sympathetic nervous system is an important reason for promoting the development of breast cancer.
ABSTRACT
Breast cancer is one of the most common malignant tumors in women,the trend of young women with breast cancer in China is obvious.Some young patients in the diagnosis of breast cancer are unmarried,so they have a certain degree of expectation of the protection of ovarian function and reali-zation of the right.In the treatment of breast cancer,the doctors should understand the effects to gonadal function.In chemotherapy,for some patients with ovarian function inhibitors have a protective effect to o-varian fuction.At present,there are some methods to protect the reproductive function,such as cryopreser-vation of oocytes,cryopreservation of embryos and preservation of ovarian tissues and so on.But thoses methods for the protection of ovarian function and reproductive function are not yet perfect.